1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD81 (65195-1-Ig, Clone:5A6) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD81 (65195-1-Ig, Clone:5A6) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD81 (CL488-65195, Clone:5A6) (red), or Mouse IgG1 Isotype Control (blue). Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD81 (CL488-65195, Clone:5A6), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD81 (CL647-65195, Clone:5A6) (red) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD81 (CL647-65195, Clone:5A6) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.
1X10^6 Balb/c mouse bone marrow cells were surface co-stained with 0.25 ug APC Anti-Mouse Ly-6G/Ly-6C (Gr-1) (RB6-8C5) and 0.25 ug FITC Plus Anti-Mouse CD11b (FITC-65055, Clone: M1/70) or 0.25 ug FITC Plus Rat IgG2b Isotype Control (LTF-2). Cells were not fixed.
1X10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.5 ug FITC Anti-Mouse CD80 (B7-1) (FITC-65076, Clone:16-10A1) (red), or 0.5 ug Isotype Control. Cells were not fixed.
1X10^6 C57BL/6 mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) and 0.5 ug FITC Anti-Mouse CD3ε (FITC-65060, Clone:145-2C11) or 0.5 ug FITC Armenian Hamster IgG Isotype Control (PIP). Cells were not fixed.
1X10^6 PHA-stimulated (5 ug/ml, 3 days) human peripheral blood lymphocytes were surface stained with 5 ul APC Anti-Human PD-L1 (B7-H1) (APC-65081, Clone: 29E.2A3) (blue) or isotype control antibody (black). Cells were not fixed.
1X10^6 mouse splenocytes were surface stained with APC Anti-Mouse CD4 (GK1.5) (APC-65104, Clone: GK1.5) and 1 ug FITC Plus Anti-Mouse CD3 (FITC-65077, Clone: 17A2) or 1 ug isotype control. Cells were not fixed.
